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Image Search Results
Journal: Cell Death & Disease
Article Title: Specific deletion of protein phosphatase 6 catalytic subunit in Sertoli cells leads to disruption of spermatogenesis
doi: 10.1038/s41419-021-04172-y
Figure Lengend Snippet: A β-Catenin (a marker of adherens junctions, green) immunofluorescence of Ppp6c WT and Ppp6c cKO mice testes. Scale bar: (left) 50 μm, (right) 20 μm. B ZO2 (a marker of tight junctions, green) immunofluorescence of Ppp6c WT and Ppp6c cKO mice testes. Scale bar: (left) 50 μm, (right) 20 μm. C TEX14 (a marker of a marker of testicular intercellular junctions, green) and β-actin (red) immunofluorescence of Ppp6c WT and Ppp6c cKO mice testes. Scale bar: (left) 50 μm, (right) 20 μm. D Vimentin (a marker for Sertoli apical extensions, green) immunofluorescence of Ppp6c WT and Ppp6c cKO mice testes. Scale bar: (left) 50 μm, (right) 20 μm. Nuclei are stained with DAPI.
Article Snippet: PPP6C antibody (rabbit, A300-844A; Bethyl Laboratories, Inc.); SYCP3 antibody (rabbit, NB300-231; Novus Biologicals); α-tubulin antibody (rabbit, 2144; Cell Signaling Technology, Inc.); β-actin antibody (mouse, 3700; Cell Signaling Technology, Inc.); Phospho-β-Catenin (Ser552) (D8E11) antibody (rabbit, 5651; Cell Signaling Technology, Inc.); SYCP3 antibody (mouse, sc-74569; Santa Cruz); γH2AX antibody (rabbit, 9718; Cell Signaling Technology, Inc.); MVH antibody (mouse, ab27591; abcam); SYCP1 antibody (rabbit, ab15090; abcam); SOX9 antibody (rabbit, AB5535, Sigma-Aldrich); PLZF antibody (goat, AF2944, R&D Systems);
Techniques: Marker, Immunofluorescence, Staining
Journal: Cell Death & Disease
Article Title: Specific deletion of protein phosphatase 6 catalytic subunit in Sertoli cells leads to disruption of spermatogenesis
doi: 10.1038/s41419-021-04172-y
Figure Lengend Snippet: A Western blotting analysis of p-β-catenin S552 protein in Ppp6c WT and Ppp6c cKO Sertoli cells of mice. β-actin was detected as an internal control. At least 3 mice of each genotype were used for analysis. B A schematic diagram of in vitro phosphatase assays which were performed by using purified PPP6C (WT or D84N) and CTNNB1. C PPP6C (WT or D84N) was incubated with CTNNB1 to perform the in vitro phosphatase assays. Immunoblotting of the assay products exhibited an effective elimination of phospho-β-catenin (S552) by PPP6C, but not by the phosphatase-dead PPP6C (D84N). D β-Catenin immunofluorescence of Ppp6c WT and Ppp6c cKO Sertoli cells of mice. Scale bar: (top) 50 μm, (bottom) 10 μm.
Article Snippet: PPP6C antibody (rabbit, A300-844A; Bethyl Laboratories, Inc.); SYCP3 antibody (rabbit, NB300-231; Novus Biologicals); α-tubulin antibody (rabbit, 2144; Cell Signaling Technology, Inc.); β-actin antibody (mouse, 3700; Cell Signaling Technology, Inc.); Phospho-β-Catenin (Ser552) (D8E11) antibody (rabbit, 5651; Cell Signaling Technology, Inc.); SYCP3 antibody (mouse, sc-74569; Santa Cruz); γH2AX antibody (rabbit, 9718; Cell Signaling Technology, Inc.); MVH antibody (mouse, ab27591; abcam); SYCP1 antibody (rabbit, ab15090; abcam); SOX9 antibody (rabbit, AB5535, Sigma-Aldrich); PLZF antibody (goat, AF2944, R&D Systems);
Techniques: Western Blot, In Vitro, Purification, Incubation, Immunofluorescence